celltiterglo® luminescent cell viability assay  (Promega)

Article Title: Therapeutic Targeting of Oncogene-induced Transcription-Replication Conflicts in Pancreatic Ductal Adenocarcinoma.
Article Snippet: .. Cell viability was determined using the CellTiterGlo Luminescent Cell Viability Assay (Promega), according to the manufacturer’s instructions. ..

Article Title: Stabilized BCL9 peptides for treatment of aberrant Wnt signaling
Article Snippet: .. TABLE 3 Additional stabilized polypeptides derived from the HD domain of BCL9 protein SEQ Corresponding ID Amino Acid Sequence position within N-terminus C-terminus NO: (WX No.) BCL9 Modificiation Modification 108 LRXaa1IQRXaa2L 366-373 Ac 2-Nal-β- (WX-021) Ala-β-Ala- NH2 109 LRXaa1IQRXaa2L 366-373 Ac ß-Ala-β- (WX-022) Ala-NH2 110 LRXaa1IQRXaa2L 366-373 Ac 2-Nal-NH2 (WX-023) 111 LQTLRXaa1IQRXaa2L 363-373 Ac 2-Nal-NH2 (WX-024) The additional polypeptides were tested in a CellTiterGlo luminescent cell viability assay (Promega). ..

Article Title: Nanomaterials Trigger Functional Anti-Tumoral Responses in Primary Human Immune Cells.
Article Snippet: .. Immune cells viability was measured at the endpoint using the CellTiterGlo luminescent cell viability assay (Promega #G7570). ..

Article Title: Applying a polysaccharide lyase from Stenotrophomonas maltophilia to disrupt alginate exopolysaccharide produced by Pseudomonas aeruginosa clinical isolates
Article Snippet: .. CellTiterGlo Luminescent Cell Viability Assay (Promega) was conducted and showed that Smlt1473 is non-cytotoxic to BEAS-2B cells. ..

Article Title: Linear peptides from the venom of large marsh horsefly (Tabanus autumnalis) larvae facilitate prey capture and defense.
Article Snippet: Ionomycin (Cayman Chemical, Ann Arbor, MI, USA) was used as a 201 positive control. .. After incubation for 48 h, cell viability was quantified using the CellTiterGlo 202 Luminescent Cell Viability Assay (Promega, Walldorf, Germany). ..

Article Title: Trans-Mediated, Cis-Inhibited Paradoxal Activity of Clostridium perfringens Enterotoxin (c-CPE) in Modulating Epithelial Permeability.
Article Snippet: In the context of transdermal delivery, favoring the drug permeability of epithelia through convenient formulations would open new opportunities for local versus systemic drug delivery, envisaging higher patient comfort and an enhanced therapeutic effect.. Ligands of tight junctions are interesting agents that enhance epithelial permeability by relaxing the protein complexes that form them.. The C-terminal domain of Clostridium perfringens enterotoxin (c-CPE), which binds claudins, one of the tight junction (TJ) components, has been explored here as a functional domain in modular recombinant proteins, to evaluate its ability to selfpromote its paracellular epithelial passage in a Caco-2 cell monolayer model. c-CPE-containing fusion proteins bind cells in the absence of internalization and cytotoxicity and support the passage, in trans, of other fusion proteins devoid of c-CPE.

Article Title: Cellular interactions within the immune microenvironment underpins resistance to cell cycle inhibition in breast cancers.
Article Snippet: To assess T cell proliferation in the absence of cancer cells, activated CD8 + T cells were cultured in RPMI-1640 + 10% hiFBS + 1x antibioticantimycotic with control (0.1% DMSO), 0.5-5 ng/mL IL-15 (R&D Systems), 1 μM ribociclib (Selleck Chemicals), or combinations 0.5-5 ng/ mL IL-15 + 1 μM ribociclib in ULA spheroid plates. .. T cell growth was monitored using Cytation 5 by brightfield imaging every 12 h. After 163 h, proliferation was assessed by measuring total ATP using the CellTiterGlo Luminescent Cell Viability Assay (Promega Corporation). ..

Article Title: Stabilized BCL9 peptides for treatment of aberrant Wnt signaling
Article Snippet: .. TABLE 3 Additional stabilized polypeptides derived from the HD domain of BCL9 protein SEQ Corresponding ID Amino Acid Sequence position within N-terminus C-terminus NO: (WX No.) BCL9 Modificiation Modification 108 LRXaa1IQRXaa2L 366-373 Ac 2-Nal-β- (WX-021) Ala-β-Ala- NH2 109 LRXaa1IQRXaa2L 366-373 Ac ß-Ala-β- (WX-022) Ala-NH2 110 LRXaa1IQRXaa2L 366-373 Ac 2-Nal-NH2 (WX-023) 111 LQTLRXaa1IQRXaa2L 363-373 Ac 2-Nal-NH2 (WX-024) The additional polypeptides were tested in a CellTiterGlo luminescent cell viability assay (Promega). ..

Article Title: Stabilized BCL9 peptides for treatment of aberrant Wnt signaling
Article Snippet: .. TABLE 3 Additional stabilized polypeptides derived from the HD domain of BCL9 protein SEQ Corresponding ID Amino Acid Sequence position within N-terminus C-terminus NO: (WX No.) BCL9 Modificiation Modification 108 LRXaa1IQRXaa2L 366-373 Ac 2-Nal-β- (WX-021) Ala-β-Ala- NH2 109 LRXaa1IQRXaa2L 366-373 Ac ß-Ala-β- (WX-022) Ala-NH2 110 LRXaa1IQRXaa2L 366-373 Ac 2-Nal-NH2 (WX-023) 111 LQTLRXaa1IQRXaa2L 363-373 Ac 2-Nal-NH2 (WX-024) The additional polypeptides were tested in a CellTiterGlo luminescent cell viability assay (Promega). ..

Article Title: Stabilized BCL9 peptides for treatment of aberrant Wnt signaling
Article Snippet: .. TABLE 3 Additional stabilized polypeptides derived from the HD domain of BCL9 protein SEQ Corresponding ID Amino Acid Sequence position within N-terminus C-terminus NO: (WX No.) BCL9 Modificiation Modification 108 LRXaa1IQRXaa2L 366-373 Ac 2-Nal-β- (WX-021) Ala-β-Ala- NH2 109 LRXaa1IQRXaa2L 366-373 Ac ß-Ala-β- (WX-022) Ala-NH2 110 LRXaa1IQRXaa2L 366-373 Ac 2-Nal-NH2 (WX-023) 111 LQTLRXaa1IQRXaa2L 363-373 Ac 2-Nal-NH2 (WX-024) The additional polypeptides were tested in a CellTiterGlo luminescent cell viability assay (Promega). ..

Article Title: Minimizing DNA trapping while maintaining activity inhibition via selective PARP1 degrader.
Article Snippet: The cytotoxic effects of 180055 on cells were assessed using the CellTiterGlo (CTG) luminescent cell viability assay (Promega).

Article Title: Linear peptides from the venom of large marsh horsefly (Tabanus autumnalis) larvae facilitate prey capture and defense.
Article Snippet: Ionomycin (Cayman Chemical, Ann Arbor, MI, USA) was used as a 201 positive control. .. After incubation for 48 h, cell viability was quantified using the CellTiterGlo 202 Luminescent Cell Viability Assay (Promega, Walldorf, Germany). ..

Article Title: Trans-Mediated, Cis-Inhibited Paradoxal Activity of Clostridium perfringens Enterotoxin (c-CPE) in Modulating Epithelial Permeability.
Article Snippet: In the context of transdermal delivery, favoring the drug permeability of epithelia through convenient formulations would open new opportunities for local versus systemic drug delivery, envisaging higher patient comfort and an enhanced therapeutic effect.. Ligands of tight junctions are interesting agents that enhance epithelial permeability by relaxing the protein complexes that form them.. The C-terminal domain of Clostridium perfringens enterotoxin (c-CPE), which binds claudins, one of the tight junction (TJ) components, has been explored here as a functional domain in modular recombinant proteins, to evaluate its ability to selfpromote its paracellular epithelial passage in a Caco-2 cell monolayer model. c-CPE-containing fusion proteins bind cells in the absence of internalization and cytotoxicity and support the passage, in trans, of other fusion proteins devoid of c-CPE.

Article Title: Cellular interactions within the immune microenvironment underpins resistance to cell cycle inhibition in breast cancers.
Article Snippet: To assess T cell proliferation in the absence of cancer cells, activated CD8 + T cells were cultured in RPMI-1640 + 10% hiFBS + 1x antibioticantimycotic with control (0.1% DMSO), 0.5-5 ng/mL IL-15 (R&D Systems), 1 μM ribociclib (Selleck Chemicals), or combinations 0.5-5 ng/ mL IL-15 + 1 μM ribociclib in ULA spheroid plates. .. T cell growth was monitored using Cytation 5 by brightfield imaging every 12 h. After 163 h, proliferation was assessed by measuring total ATP using the CellTiterGlo Luminescent Cell Viability Assay (Promega Corporation). ..